NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals
نویسندگان
چکیده
Abstract The color of flowers is one the main characteristics adopted for plants to attract pollinators ensure reproductive success plant, they are also important in their ornamental appeal Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis loss- and gain- tissue relative wild-type narcissus showed was mainly involved flavonol phenylpropanoid metabolic pathways. Biochemical evidences dual-luciferase activity chromatin immunoprecipitation assay supported that MYB12 directly bound promoters NtFLS , NtLAR, NtDFR were cloned by genome walking assay, activated NtLAR expression but repressed expression. More interestingly, can interact with NtbHLH1 NtWD40-1 proteins via R3 domain selected transcriptome-based WGCNA confirmed yeast two hybrid, bimolecular fluorescence complementation coimmunoprecipitation assay. Interaction forming MYB-bHLH-WD40 triplex specially NtANS promoted (pro)anthocyanin accumulation, while alone accumulated flavonols, These results indicated or NtMYB12-bHLH1-WD40-1 requires competition metabolism fluxes between biosynthesis. Graphical dually functions on proanthocyanin biogenesis physically binding promoter activating biosynthesis NtMYB12-NtWD40-NtbHLH (MBW) Requirement MBW complex anthocyanin colorized petal traits.
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ژورنال
عنوان ژورنال: Molecular Horticulture
سال: 2023
ISSN: ['2730-9401']
DOI: https://doi.org/10.1186/s43897-023-00050-7